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Top Forums Shell Programming and Scripting Changing from FASTA to PHYLIP format Post 302498206 by Xterra on Sunday 20th of February 2011 12:57:12 PM
Old 02-20-2011
Changing from FASTA to PHYLIP format

I really need some help with this task. I have a bunch of FASTA files with hundreds of DNA sequences that look like this:
Code:
>SeqID1
AACCATGACAGAGGAGATGTGAACAGATAGAGGGATGACAGATGACAGATAGACCCAGAC
TGACAGGTTCAAAGGCTGCAGTGCAGTGACGTGACGATTT
>Sequence 22
AACCATGACAGAGGAGATGTGAACAGATAGAGGGATGACAGATGACAGATAGACCCAGAC
TGACAGGTTCAAAGGCTGCAGTGCAGTGACGTGACGATTT
>Seq-39
AACCATGACAGAGGAGATGTGAACAGATAGAGGGATGACAGATGACAGATAGACCCAGAC
TGACAGGTTCAAAGGCTGCAGTGCAGTGACGTGACGATTT

And I need to change the format (Phylip) so they can look like this:
Code:
3 100 
SeID1_____AACCATGACAGAGGAGATGTGAACAGATAGAGGGATGACAGATGACAGATAGACCCAGACTGACAGGTTCAAAGGCTGCAGTGCAGTGACGTGACGATTT 
Sequence__AACCATGACAGAGGAGATGTGAACAGATAGAGGGATGACAGATGACAGATAGACCCAGACTGACAGGTTCAAAGGCTGCAGTGCAGTGACGTGACGATTT 
Seq-39____AACCATGACAGAGGAGATGTGAACAGATAGAGGGATGACAGATGACAGATAGACCCAGACTGACAGGTTCAAAGGCTGCAGTGCAGTGACGTGACGATTT

The first number at the very top is the number of sequences followed by the length of the sequences.
The first column is the Sequence ID that needs to be 8 characters long followed by 2 blank spaces and then the actual sequence. If the SequenceID is longer than 8 characters, then the extra characters should be removed. If the SequenceID is shorter than 8, blank spaces should be added to keep the length to 8. In my example I have added underscores to keep the sequences aligned and accurately reflect how the output file should look but in the outfile they should be blank spaces.
Any help will be greatly appreciate it!
 

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FASTX_CLIPPER(1)						   User Commands						  FASTX_CLIPPER(1)

NAME
fastx_clipper - FASTA/Q Clipper DESCRIPTION
usage: fastx_clipper [-h] [-a ADAPTER] [-D] [-l N] [-n] [-d N] [-c] [-C] [-o] [-v] [-z] [-i INFILE] [-o OUTFILE] Part of FASTX Toolkit 0.0.13.2 by A. Gordon (gordon@cshl.edu) [-h] = This helpful help screen. [-a ADAPTER] = ADAPTER string. default is CCTTAAGG (dummy adapter). [-l N] = discard sequences shorter than N nucleotides. default is 5. [-d N] = Keep the adapter and N bases after it. (using '-d 0' is the same as not using '-d' at all. which is the default). [-c] = Discard non-clipped sequences (i.e. - keep only sequences which contained the adapter). [-C] = Discard clipped sequences (i.e. - keep only sequences which did not contained the adapter). [-k] = Report Adapter-Only sequences. [-n] = keep sequences with unknown (N) nucleotides. default is to discard such sequences. [-v] = Verbose - report number of sequences. If [-o] is specified, report will be printed to STDOUT. If [-o] is not specified (and output goes to STDOUT), report will be printed to STDERR. [-z] = Compress output with GZIP. [-D] = DEBUG output. [-M N] = require minimum adapter alignment length of N. If less than N nucleotides aligned with the adapter - don't clip it. [-i INFILE] = FASTA/Q input file. default is STDIN. [-o OUTFILE] = FASTA/Q output file. default is STDOUT. SEE ALSO
The quality of this automatically generated manpage might be insufficient. It is suggested to visit http://hannonlab.cshl.edu/fastx_toolkit/commandline.html to get a better layout as well as an overview about connected FASTX tools. fastx_clipper 0.0.13.2 May 2012 FASTX_CLIPPER(1)
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